Sunday, July 20, 2008

Microscopic research in 1664

Excerpts from: MICROGRAPHIA: OR SOME Physiological Descriptions OF MINUTE BODIES MADE BY MAGNIFYING GLASSES WITH OBSERVATIONS and INQUIRIES thereupon. By R. HOOKE, Fellow of the ROYAL SOCIETY.
Non possis oculo quantum contendere Linceus,
Non tamen idcirco contemnas Lippus inungi. Horat. Ep. lib. 1.
LONDON, Printed by Jo. Martyn, and Ja. Allestry, Printers to the ROYAL SOCIETY, and are to be sold at their Shop at the Bell in S. Paul's Church-yard. M DC LX V.

TO THE KING.
SIR,

I Do here most humbly lay this small Present at Your Majesties Royal feet. And though it comes accompany'd with two disadvantages, the meanness of the Author, and of the Subject; yet in both I am incouraged by the greatness of your Mercy and your Knowledge. By the one I am taught, that you can forgive the most presumptuous Offendors: And by the other, that you will not esteem the least work of Nature, or Art, unworthy your Observation. Amidst the many felicities that have accompani'd your Majesties happy Restauration and Government, it is none of the least considerable that Philosophy and Experimental Learning have prosper'd under your Royal Patronage. And as the calm prosperity of your Reign has given us the leisure to follow these Studies of quiet and retirement, so it is just, that the Fruits of them should, by way of acknowledgement, be return'd to your Majesty. There are, Sir, several other of your Subjects, of your Royal Society, now busie about Nobler matters: The Improvement of Manufactures and Agriculture, the Increase of Commerce, the Advantage of Navigation: In all which they are assisted by your Majesties Incouragement and Example. Amidst all those greater Designs, I here presume to bring in that which is more proportionable to the smalness of my Abilities, and to offer some of the least of all visible things, to that Mighty King, that has establisht an Empire over the best of all Invisible things of this World, the Minds of Men.

Your Majesties most humble and most obedient Subject and Servant,
ROBERT HOOKE.
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From hence the World may be assisted with variety of Inventions, new matter for Sciences may be collected, the old improv'd, and their rust rubb'd away; and as it is by the benefit of Senses that we receive all our Skill in the works of Nature, so they also may be wonderfully benefited by it, and may be guided to an easier and more exact performance of their Offices; 'tis not unlikely, but that we may find out wherein our Senses are deficient, and as easily find wayes of repairing them.
The Indeavours of Skilful men have been most conversant about the assistance of the Eye, and many noble Productions have followed upon it; and from hence we may conclude, that there it a way open'd for advancing the operations, not only of all the other Senses, but even of the Eye it self; that which has been already done ought not to content us, but rather to incourage us to proceed further, and to attempt greater things in the same, and different wayes.
'Tis not unlikely, but that there may be yet invented several other helps for the eye, at much exceeding those already found, as those do the bare eye, such as by which we may perhaps be able to discover living Creatures in the Moon, or other Planets, the figures of the compounding Particles of matter, and the particular Schematisms and Textures of Bodies.
And as Glasses have highly promoted our seeing, so 'tis not improbable, but that there may be found many Mechanical Inventions to improve our other Senses, of hearing, smelling, tasting, touching. 'Tis not impossible to hear a whisper a furlongs distance, it having been already done; and perhaps the nature of the thing would not make it more impossible, though that furlong should be ten times multiply'd. And though some famous Authors have affirm'd it impossible to hear through the thinnest plate of Muscovy-glass; yet I know a way, by which 'tis easie enough to hear one speak through a wall a yard thick. It has not been yet thoroughly examin'd, how far Otocousticons may be improv'd, nor what other wayes there may be of quickning our hearing, or conveying sound through other bodies then the Air: for that that it not the only medium, I can assure the Reader, that I have, by the help of a distended wire, propagated the sound to a very considerable distance in an instant, or with as seemingly quick a motion as that of light, at least, incomparably swifter then that, which at the same time was propagated through the Air; and this not only in a straight line, or direct, but in one bended in many angles.

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[Click on images to enlarge]
First, for Microscopes (where the Object we view is near and within our power) the best way of making it appear bright in the Glass, is to cast a great quantity of light on it by means of convex glasses, for thereby, though the aperture be very small, yet there will throng in through it such multitudes, that an Object will by this means indure to be magnifi'd as much again as it would be without it. The way for doing which is this. I make choice of some Room that has only one window open to the South, and at about three or four foot distance from this Window, on a Table, I place my Microscope, and then so place either a round Globe of Water, or a very deep clear plano convex Glass (whose convex side is turn'd towards the Window) that there is a great quantity of Rayes collected and thrown upon the Object: Or if the Sun shine, I place a small piece of oyly Paper very near the Object, between that and the light; then with a good large Burning-Glass I so collect and throw the Rayes on the Paper, that there may be a very great quantity of light pass through it to the Object; yet I so proportion that light, that it may not singe or burn the Paper. Instead of which Paper there may be made use of a small piece of Looking-glass plate, one of whose sides is made rough by being rubb'd on a flat Tool with very find sand, this will, if the heat be leisurely cast on it, indure a much greater degree of heat, and consequently very much augment a convenient light. By all which means the light of the Sun, or of a Window, may be so cast on an Object, as to make it twice as light as it would otherwise be without it, and that without any inconvenience of glaring, which the immediate light of the Sun is very apt to create in most Objects; for by this means the light is so equally diffused, that all parts are alike inlightned; but when the immediate light of the Sun falls on it, the reflexions from some few parts are so vivid, that they drown the appearance of all the other, and are themselves also, by reason of the inequality of light, indistinct, and appear only radiant spots.
But because the light of the Sun, and also that of a Window, is in a continual variation, and so many Objects cannot be view'd long enough by them to be throughly examin'd; besides that, oftentimes the Weather is so dark and cloudy, that for many dayes together nothing can be view'd: And because also there are many Objects to be met with in the night, which cannot so conveniently be kept perhaps till the day, therefore to procure and cast a sufficient quantity of light on an Object in the night, I thought of, and often used this, Expedient.
I procur'd me a small Pedestal, such as is describ'd in the fifth Figure of the first Scheme on the small Pillar AB, of which were two movable Armes CD, which by means of the Screws EF, I could fix in any part of the Pillar; on the undermost of these I plac'd a pretty large Globe of Glass G, fill'd with exceeding clear Brine, stopt, inverted, and fixt in the manner visible in the Figure; out of the side of which Arm proceeded another Arm H, with many joynts; to the end of which was fastned a deep plain Convex glass I, which by means of this Arm could be moved too and fro, and fixt in any posture. On the upper Arm was placed a small Lamp K, which could be to mov'd upon the end of the Arm, as to be set in a fit posture to give light through the Ball: By means of this Instrument duly plac'd, as is exprest in the Figure, with the small flame of a Lamp may be cast as great and convenient a light on the Object as it will well indure; and being always constant, and to be had at any time, I found most proper for drawing the representations of those small Objects I had occasion to observe.
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Observ. I. Of the Point of a sharp small Needle.
As in Geometry, the most natural way of beginning is from a Mathematical point; so is the same method in Observations and Natural history the most genuine, simple, and instructive. We must first endevour to make letters, and draw single strokes true, before we venture to write whole Sentences, or to draw large Pictures. And in Physical Enquiries, we must endevour to follow Nature in the more plain and easie ways she treads in the most simple and uncompounded bodies, to trace her steps, and be acquainted with her manner of walking there, before we venture our selves into the multitude of meanders she has in bodies of a more complicated nature; lest, being unable to distinguish and judge of our way, we quickly lose both Nature our Guide, and our selves too, and are left to wander in the labyrinth of groundless opinions; wanting both judgment, that light, and experience, that clew, which should direct our proceedings.
We will begin these our Inquiries therefore with the Observations of Bodies of the most simple nature first, and so gradually proceed to those of a more compounded one. In prosecution of which method, we shall begin with a Physical point; of which kind the Point of a Needle is commonly reckon'd for one; and is indeed, for the most part, made so sharp, that the naked eye cannot distinguish any parts of it: It very easily pierces, and makes its way through all kind of bodies softer then it self: But if view'd with a very good Microscope, we may find that the top of a Needle (though as to the sense very sharp) appears a broad, blunt, and very irregular end; not resembling a Cone, as is imagin'd, but onely a piece of a tapering body, with a great part of the top remov'd, or deficient. The Points of Pins are yet more blunt, and the Points of the most curious Mathematical Instruments do very seldome arrive at so great a sharpness; how much therefore can be built upon demonstrations made onely by the productions of the Ruler and Compasses, he will be better able to consider that shall but view those points and lines with a Microscope.
Now though this point be commonly accounted the sharpest (whence when we would express the sharpness of a point the most superlatively, we say, As sharp as a Needle) yet the Microscope can afford us hundreds of Instances of Points many thousand times sharper: such as those of the hairs, and bristles, and claws of multitudes of Insects; the thorns, or crooks, or hairs of leaves, and other small vegetables; nay, the ends of the stiriæ or small parallelipipeds of Amianthus, and alumen plumosum; of many of which, though the Points are so sharp as not to be visible, though view'd with a Microscope (which magnifies the Object, in bulk, above a million of times) yet I doubt not, but were we able practically to make Microscopes according to the theory of them, we might find hills, and dales, and pores, and a sufficient bredth, or expansion, to give all those parts elbow-room, even in the blunt top of the very Point of any of these so very sharp bodies. For certainly the quantity or extension of any body may be Divisible in infinitum, though perhaps not the matter.
But to proceed: The Image we have here exhibited in the first Figure, was the top of a small and very sharp Needle, whose point aa nevertheless appear'd through the Microscope above a quarter of an inch broad, not round nor flat, but irregular and uneven; so that it seem'd to have been big enough to have afforded a hundred armed Mites room enough to be rang'd by each other without endangering the breaking one anothers necks, by being thrust off on either side. The surface of which, though appearing to the naked eye very smooth, could not nevertheless hide a multitude of holes and scratches and ruggednesses from being discover'd by the Microscope to invest it, several of which inequalities (as A, B, C, seem'd holes made by some small specks of Rust; and D some adventitious body, that stuck very close to it) were casual. All the rest that roughen the surface, were onely so many marks of the rudeness and bungling of Art. So unaccurate is it, in all its productions, even in those which seem most neat, that if examin'd with an organ more acute then that by which they were made, the more we see of their shape, the less appearance will there be of their beauty: whereas in the works of Nature, the deepest Discoveries shew us the greatest Excellencies. An evident Argument, that he that was the Author of all these things, was no other then Omnipotent; being able to include as great a variety of parts and contrivances in the yet smallest Discernable Point, as in those vaster bodies (which comparatively are called also Points) such as the Earth, Sun, or Planets. Nor need it seem strange that the Earth it self may be by Analogie call'd a Physical Point: For as its body, though now so near us as to fill our eys and fancies with a sense of the vastness of it, may by a little Distance, and some convenient Diminishing Glasses, be made vanish into a scarce visible Speck, or Point (as I have often try'd on the Moon, and (when not too bright) on the Sun it self.) So, could a Mechanical contrivance succesfully answer our Theory, we might see the least spot as big as the Earth it self; and Discover, as Des Cartes also conjectures (Diop. ch. 10. § 9.), as great a variety of bodies in the Moon, or Planets, as in the Earth.
But leaving these Discoveries to future Industries, we shall proceed to add one Observation more of a point commonly so call'd, that is, the mark of a full stop, or period. And for this purpose I observed many both printed ones and written; and among multitudes I found few of them more round or Schem. 2.
Fig. 3. regular then this which I have delineated in the third figure of the second Scheme, but very many abundantly more disfigur'd; and for the most part if they seem'd equally round to the eye, I found those points that had been made by a Copper-plate, and Roll-press, to be as misshapen as those which had been made with Types, the most curious and smothly engraven strokes and points, looking but as so many furrows and holes, and their printed impressions, but like smutty daubings on a matt or uneven floor with a blunt extinguisht brand or stick's end. And as for points made with a pen they were much more ragged and deformed. Nay, having view'd certain pieces of exceeding curious writing of the kind (one of which in the bredth of a two-pence compris'd the Lords prayer, the Apostles Creed, the ten Commandments, and about half a dozen verses besides of the Bible, whose lines were so small and near together, that I was unable to number them with my naked eye,) a very ordinary Microscope, I had then about me, inabled me to see that what the Writer of it had asserted was true, but withall discover'd of what pitifull bungling scribbles and scrawls it was compos'd, Arabian and China characters being almost as well shap'd, yet thus much I must say for the Man, that it was for the most part legible enough, though in some places there wanted a good fantsy well preposest to help one through. If this manner of small writing were made easie and practicable (and I think I know such a one, but have never yet made tryal of it, whereby one might be inabled to write a great deale with much ease, and accurately enough in a very little roome) it might be of very good use to convey secret Intelligence without any danger of Discovery or mistrusting. But to come again to the point. The Irregularities of it are caused by three or four coadjutors, one of which is, the uneven surface of the paper, which at best appears no smother then a very course piece of shag'd cloth, next the irregularity of the Type or Ingraving, and a third is the rough Daubing of the Printing-Ink that lies upon the instrument that makes the impression, to all which, add the variation made by the Different lights and shadows, and you may have sufficient reason to guess that a point may appear much more ugly then this, which I have here presented, which though it appear'd through the Microscope gray, like a great splatch of London dirt, about three inches over; yet to the naked eye it was black and no bigger then that in the midst of the Circle A. And could I have found Room in this Plate to have inserted an O you should have seen that the letters were not more distinct then the points of Distinction, nor a drawn circle more exactly so, then we have now shown a point to be a point.
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Observ. II. Of the Edge of a Razor.
The sharpest Edge hath the same kind of affinity to the sharpest Point in Physicks, as a line hath to a point in Mathematicks; and therefore the Treaty concerning this, may very properly be annexed to the former. A Razor doth appear to be a Body of a very neat and curious aspect, till more closely viewed by the Microscope, and there we may observe its very Edge to be of all kind of shapes, except what it should be. For examining that of a very sharp one, I could not find that any part of it had any thing of sharpness in it; but it appeared a rough surface of a very considerable bredth from side to side, the narrowest part not seeming thinner then the back of a pretty thick Knife. Nor is't likely that it should appear any otherwise, since as we just now shew'd that a point appear'd a circle, 'tis rational a line should be a parallelogram.
Now for the drawing this second Figure (which represents a part of the Edge about half a quarter of an inch long of a Razor well set) I so plac'd it between the Object-glass & the light, that there appear'd a reflection from the very Edge, represented by the white line abcdef. In which you may perceive it to be somewhat sharper then elsewhere about d, to be indented or pitted about b, to be broader and thicker about c, and unequal and rugged about e, and pretty even between ab and ef. Nor was that part of the Edge ghik so smooth as one would imagine so smooth bodies as a Hone and Oyl should leave it; for besides those multitudes of scratches, which appear to have raz'd the surface ghik, and to cross each other every way which are not half of them exprest in the Figure, there were several great and deep scratches, or furrows, such as gh and ik, which made the surface yet more rugged, caus'd perhaps by some small Dust casually falling on the Hone, or some harder or more flinty part of the Hone it self. The other part of the Razor ll, which is polish'd on a grinding-stone, appear'd much rougher then the other, looking almost like a plow'd field, with many parallels, ridges, and furrows, and a cloddy, as 'twere, or an uneven surface: nor shall we wonder at the roughnesses of those surfaces, since even in the most curious wrought Glasses for Microscopes, and other Optical uses, I have, when the Sun has shone well on them, discover'd their surface to be variously raz'd or scratched, and to consist of an infinite of small broken surfaces, which reflect the light of very various and differing colours. And indeed it seems impossible by Art to cut the surface of any hard and brittle body smooth, since Putte, or even the most curious Powder that can be made use of, to polish such a body, must consist of little hard rough particles, and each of them must cut its way, and consequently leave some kind of gutter or furrow behind it. And though Nature does seem to do it very readily in all kinds of fluid bodies, yet perhaps future observators may discover even these also rugged; it being very probable, as I elsewhere shew, that fluid bodies are made up of small solid particles variously and strongly mov'd, and may find reason to think there is scarce a surface in rerum naturâ perfectly smooth. The black spot mn, I ghess to be some small speck of rust, for that I have oft observ'd to be the manner of the working of Corrosive Juyces. To conclude, this Edge and piece of a Razor, if it had been really such as it appear'd through the Microscope, would scarcely have serv'd to cleave wood, much less to have cut off the hair of beards, unless it were after the manner that Lucian merrily relates Charon to have made use of, when with a Carpenters Axe he chop'd off the beard of a sage Philosopher, whose gravity he very cautiously fear'd would indanger the oversetting of his Wherry.
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To make small shot of different sizes; Communicated by his Highness P.R.
Take Lead out of the Pig what quantity you please, melt it down, stir and clear it with an iron Ladle, gathering together the blackish parts that swim at top like scum, and when you see the colour of the clear Lead to be greenish, but no sooner, strew upon it Auripigmentum powdered according to the quantity of Lead, about as much as will lye upon a half Crown piece will serve for eighteen or twenty pound weight of some sorts of Lead; others will require more, or less. After the Auripigmentum is put in, stir the Lead well, and the Auripigmentum will flame: when the flame is over, take out some of the Lead in a Ladle having a lip or notch in the brim for convenient pouring out of the Lead, and being well warmed amongst the melted Lead, and with a stick make some single drops of Lead trickle out of the Ladle into water in a Glass, which if they fall to be round and without tails, there is Auripigmentum enough put in, and the temper of the heat is right, otherwise put in more. Then lay two bars of Iron (or some more proper Iron-tool made on purpose) upon a Pail of water, and place upon them a round Plate of Copper, of the size and figure of an ordinary large Pewter or Silver Trencher, the hollow whereof is to be about three inches over, the bottom lower then the brims about half an inch, pierced with thirty, forty, or more small holes; the smaller the holes are, the smaller the shot will be; and the brim is to be thicker then the bottom, to conserve the heat the better.
The bottom of the Trencher being some four inches distant from the water in the Pail, lay upon it some burning Coles, to keep the Lead melted upon it. Then with the hot Ladle take Lead off the Pot where it stands melted, and pour it softly upon the burning Coles over the bottom of the Trencher, and it will immediately run through the holes into the water in small round drops. Thus pour on new Lead still as fast as it runs through the Trencher till all be done; blowing now and then the Coles with hand-Bellows, when the Lead in the Trencher cools so as to stop from running.
While one pours on the Lead, another must, with another Ladle, thrusted four or five inches under water in the Pail, catch from time to time some of the shot, as it drops down, to see the size of it, and whether there be any faults in it. The greatest care is to keep the Lead upon the Trencher in the right degree of heat; if it be too cool, it will not run through the Trencher, though it stand melted upon it; and this is to be helped by blowing the Coals a little, or pouring on new Lead that is hotter: but the cooler the Lead, the larger the Shot; and the hotter, the smaller; when it it too hot, the drops will crack and fly; then you must stop pouring on new Lead, and let it cool; and so long as you observe the right temper of the heat, the Lead will constantly drop into very round Shot, without so much as one with a tail in many pounds.
When all is done, take your Shot out of the Pail of water, and put it in a Frying-pan over the fire to dry them, which must be done warily, still shaking them that they melt not; and when they are dry you may separate the small from the great, in Pearl Sives made of Copper or Lattin let into one another, into as many sizes at you please. But if you would have your Shot larger then the Trencher makes them, you may do it with a Stick, making them trickle out of the Ladle, as hath been said.
If the Trencher be but toucht a very little when the Lead stops from going through it, and be not too cool, it will drop again, but it it better not to touch it at all. At the melting of the Lead take care that there be no kind of Oyl, Grease, or the like, upon the Pots, or Ladles, or Trencher.
The Chief cause of this Globular Figure of the Shot, seems to be the Auripigmentum; for, as soon as it is put in among the melted Lead, it loses its shining brightness, contracting instantly a grayish film or skin upon it, when you scum it to make it clean with the Ladle. So that when the Air comes at the falling drop of the melted Lead, that skin constricts them every where equally: but upon what account, and whether this be the true cause, is left to further disquisition.

Much after this same manner, when the Air is exceeding cold through which it passes; do we find the drops of Rain, falling from the Clouds, congealed into round Hail-stones by the freezing Ambient.
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Observ. LIII. Of a Flea.
The strength and beauty of this small creature, had it no other relation at all to man, would deserve a description.
For its strength, the Microscope is able to make no greater discoveries of it then the naked eye, but onely the curious contrivance of its leggs and joints, for the exerting that strength, is very plainly manifested, such as no other creature, I have yet observ'd, has any thing like it; for the joints of it are so adapted, that he can, as 'twere, fold them short one within another, and suddenly stretch, or spring them Schem. 34. out to their whole length, that is, of the fore-leggs, the part A, of the 34. Scheme, lies within B, and B within C, parallel to, or side by side each other; but the parts of the two next, lie quite contrary, that is, D without E, and E without F, but parallel also; but the parts of the hinder leggs, G, H and I, bend one within another, like the parts of a double jointed Ruler, or like the foot, legg and thigh of a man; these six leggs he clitches up altogether, and when he leaps, springs them all out, and thereby exerts his whole strength at once.
But, as for the beauty of it, the Microscope manifests it to be all over adorn'd with a curiously polish'd suit of sable Armour, neatly jointed, and beset with multitudes of sharp pinns, shap'd almost like Porcupine's Quills, or bright conical Steel-bodkins; the head is on either side beautify'd with a quick and round black eye K, behind each of which also appears a small cavity, L, in which he seems to move to and fro a certain thin film beset with many small transparent hairs, which probably may be his ears; in the forepart of his head, between the two fore-leggs, he has two small long jointed feelers, or rather smellers, MM, which have four joints, and are hairy, like those of several other creatures; between these, it has a small proboscis, or probe, NNO, that seems to consist of a tube NN, and a tongue or sucker O, which I have perceiv'd him to slip in and out. Besides these, it has also two chaps or biters PP, which are somewhat like those of an Ant, but I could not perceive them tooth'd; these were shap'd very like the blades of a pair of round top'd Scizers, and were opened and shut just after the same manner; with these Instruments does this little busie Creature bite and pierce the skin, and suck out the blood of an Animal, leaving the skin inflamed with a small round red spot. These parts are very difficult to be discovered, because, for the most part, they lye covered between the fore-legs. There are many other particulars, which, being more obvious, and affording no great matter of information, I shall pass by, and refer the Reader to the Figure.
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Observ. XX. Of blue Mould, and of the first Principles of Vegetation arising from Putrefaction.
The Blue and White and several kinds of hairy mouldy spots, which are observable upon divers kinds of putrify'd bodies, whether Animal substances, or Vegetable, such as the skin, raw or dress'd, flesh, bloud, humours, milk, green Cheese, &c. or rotten sappy Wood, or Herbs, Leaves, Barks, Roots, &c. of Plants, are all of them nothing else but several kinds of small and variously figur'd Mushroms, which, from convenient materials in those putrifying bodies, are, by the concurrent heat of the Air, excited to a certain kind of vegetation, which will not be unworthy our more serious speculation and examination, as I shall by and by shew. But, first, I must premise a short description of this Specimen, which I have added of this Tribe, in the first Figure of the XII. Scheme, which is nothing else but the appearance of a small white spot of hairy mould, multitudes of which I found to bespeck & whiten over the red covers of a small book, which, it seems, were of Sheeps skin, that being more apt to gather mould, even in a dry and clean room, then other leathers. These spots appear'd, through a good Microscope, to be a very pretty shap'd Vegetative body, which, from almost the same part of the Leather, shot out multitudes of small long cylindrical and transparent stalks, not exactly streight, but a little bended with the weight of a round and white knob that grew on the top of each of them; many of these knobs I observ'd to be very round, and of a smooth surface, such as A, A, &c. others smooth likewise, but a little oblong, as B; several of them a little broken, or cloven with chops at the top, as C; others flitter'd as 'twere, or flown all to pieces, as D, D. The whole substance of these pretty bodies was of a very tender constitution, much like the substance of the softer kind of common white Mushroms, for by touching them with a Pin, I found them to be brused and torn; they seem'd each of them to have a distinct root of their own; for though they grew neer together in a cluster, yet I could perceive each stem to rise out of a distinct part or pore of the Leather; some of these were small and short, as seeming to have been but newly sprung up, of these the balls were for the most part round, others were bigger, and taller, as being perhaps of a longer growth, and of these, for the most part, the heads were broken, and some much wasted, as E; what these heads contain'd I could not perceive; whether they were knobs and flowers, or seed cases, I am not able to say, but they seem'd most likely to be of the same nature with those that grow on Mushroms, which they did, some of them, not a little resemble.

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